Exact(5)
By selecting such a low threshold, we introduced undesirable PCR byproduct in the form of short DNAs (e.g., undigested primers, primer dimers, etc).. Had the threshold of DNA concentration been set higher prior to final pooling and/or a membrane filter been used to further purify the final pooled product, more small fragment DNAs could have been excluded.
When the morphological wavelet decomposition is used in the first stage of morphological multi-scale IQA framework, multi-scale wavelet mean squared error (MW-MSE) is calculated as weighted sum of ( mathit{mathsf{M}}mathit{mathsf{S}}{mathit{mathsf{E}}}_{mathit{mathsf{j}}mathit{mathsf{i}}} ) values for all subbands at all scales of the two wavelet representations as final pooling (11).
Single voids were stored under darkened and cooled conditions until final pooling into 24-h volumes.
Also, the threshold for including a PCR 2 product in the final pooling step was set to a DNA concentration of 14 ng/μl.
A final pooling, which contained equal quantities of each participant, was performed with all of the ligation products, and 12 cycles of PCR were performed using library amplification primers to produce the templates for deep sequencing.
Similar(55)
However, the one dark cloud is the improving Heineken form of Castres, their final pool opponents.
England are already due to face Scotland at Eden Park in their final pool fixture.
The victory in Harlem's final Pool B game sends the New Yorkers (2-1) against Worcester, Mass.
The water in the final pools is noticeably paler and clearer.
Brazil made it to the final pool, whereupon they beat Sweden 7-1 and Spain 6-1.
South Africa completed the last eight when it beat Afghanistan in the final pool-stage match.
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