Final library was purified with 1x AMPure XP beads.
Quantification and size distribution of the final library was determined as described above before sequencing.
Equal amounts of each library of 96 were combined to give the final library used for sequencing.
Different fingerprints are used to facilitate a similarity step for database mining, followed by a diversity step to assemble the final library.
The final library, which consisted of 13,769 compounds, displayed a 2.6% hit rate when screened against the μ-opioid receptor.
Small portions of transformed cells were serially diluted and titrated on SD-SCAA plates to confirm the final library sizes (108 range).
A high sensitivity DNA chip was used and 1 μl of the final library was run in the Agilent 2100 bioanalyser.
Dr. Gibarian's monologue [from the novel's sixth chapter] is the highlight of the final library scene, wherein Snaut says, "We don't need other worlds.
The final library contains about 1400 decoys for each protein.
Final library concentration was determined by fluorescent based assay on the Qubit (Life Technologies).
The concentration of final library was measured using the Agilent 2100 Bioanalyzer (Palo Alto, CA).
