Surfaces were sterilized in 70% ethanol for 1 h immediately following plasma treatment, followed by three sequential 5 s immersions in 70% ethanol, two sequential 5 s immersions in HEPES saline solution and a final 10 immersion in cell culture media prior to cell seeding.
David wants a final mad immersion in lust, which arrives, not to his surprise, with its attendant idiocies of jealousy and possessiveness.
She wants a connection to culture; David wants a final mad immersion in lust, which arrives, not to his surprise, with its attendant idiocies of jealousy and possessiveness.
Although he kept one of these paintings with him all his life, he appears to have stopped work on both around the time of his final stylistic immersion, into Surrealism.
In particular, under our experimental conditions, the immersion media was diluted, and the final concentration was 25% (v/v) to avoid further culture medium dilution and thus adverse effects on cell physiology.
Both mouse and human tissues were treated with formaldehyde to covalently link transcription factors to DNA sites of interaction by either immersion in, or perfusion with 1% final concentration formaldehyde, followed by homogenization using a manual glass cell homogenizer.
Crystals were supplemented with either PEG 400 or 5/4 PO/OH to a final concentration of 35% (vol/vol) prior to vitrification by direct immersion in liquid nitrogen.
Glycerol was used as a cryoprotectant and incrementally added to the mother liquor to a final concentration of 30% before flash-freezing the crystals by immersion in liquid nitrogen.
This study varied the first two constructs; the final construct of immersion was held constant in that each condition viewed the same large projection area, a 78-inch diagonal.
The final study used the Immersion® CyberGlove® to record participants' interactions with a prop in place of a touchscreen phone when carrying out various tasks.
