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A final extraction was done with 24:1 chloroform:isoamylalcohol. Extracted DNA was precipitated by adding 1/10 volume of 3 M sodium acetate (pH 5.5) and 2 volumes of 100%% ethanol and centrifugation for 10 min and 4 °C at 19,000 g.
Lastly, the pellet left following sodium chlorite treatment was subjected to a final extraction with 4 M KOH containing 1% (w/v) sodium borohydride to extract material that had previously been secured within the walls by lignin (4 M KOH PC).
Finally, the root of the ankylosed tooth at the time of final extraction has almost totally been replaced by bone diminishing the risk of fracture.
Data extracted were compared and discussed by the two reviewers and a final extraction form was compiled.
The third and final extraction yields blackstrap molasses, a heavy, viscous, dark-coloured product that has had all the sugar removed from it that can be separated practically by ordinary crystallization.
The final extraction conditions were concluded as follows: ethanol concentration 21.73% (w/w) and NaH2PO4 concentration 23.27% (w/w) for the ATPS, extraction temperature 95 °C, extraction time 5.8 min, solvent-to-material ratio 21 1 (mL/g) and particle size 80 mesh.
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Year-3 and final extractions took place in late May and early June 2013.
The concentration and quality of final extractions were examined using a ND-1000 UV spectrophotometer (Nanodrop, Wilmington, DE).
The final optimum extraction conditions were: headspace extraction at 70 °C during 40 min adding 200 g L−1 of NaCl.
All authors contributed to development of the final data extraction form, agreed review methodology and data extracted a share of the included studies.
Following the final organic extraction, the remaining aqueous component was further extracted twice with water, and protein removed by precipitation with 3 1 acetonitrile (extract B).
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