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Analytes were isolated through supported liquid extraction, and the final extract was analyzed via HPLC with MS/MS detection.
The extraction method comprises a combination of water and organic solvent extractions, and each final extract is assayed for absence of residual solvents.
Up to 2 g of tissue or 2 mL of urine was taken for extraction, and the final extract was concentrated to as low as 200 μL to get responses above the LOQ range.
Finally solvent was removed completely to get final extract.
The extract was concentrated using rotary evaporator (BUCHI, R-200 model, Germany) and the extraction yield was estimated from the final extract concentrate using Equation (1).
The pH of the final extract is a decisive parameter that influences the performance of the extraction procedure.
Finally, 100 μg mirex (Promochem, Wesel, Germany) was added to the final extract as an internal standard.
That's the question asked by Chris Frost, head of journalism at Liverpool John Moores university, in what will be the book's final extract.
Today's final extract from the updated edition of The phone hacking scandal: journalism on trial*, is by one of the book's editors, John Mair.
Next - the final extract: A call for innovation and radical thinking by Neil Fowler, a former editor and publisher *What do we mean by local? is edited by John Mair, Neil Fowler & Ian Reeves and published by Abramis.
The final extract was analysed by HPLC-FLD after precolumn derivatisation with ortho-phthaldialdehyde.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com