The final design was designated A-92 by the IJN and differed enough from the A-64 design of the Fusō class that it was considered a separate class.
The variables were thus transformed by means of the sum of the individual scores into the final score designated IRS.
A gene was discarded from the final results (designated NA in Table S1) if it was replicated in less than 8 spots after filtering of the data.
The final construct designated pYEX- Lca-KPVQ was transformed for functional expression into the ole1 elo1 yeast.
A final construct, designated C3A, was generated where protein dimerisation was severely impaired by mutation of all three extracellular cysteines to alanine residues.
When an isolated rat heart recovered its ability to beat, the perfusate was continued for an additional 25 minutes (to the final time designated as Te).
The final constructs designated as pH7WG2 GNT2 and pK7FWG2 GNT2, were verified by sequencing (Applied Biosystems 377 gel sequencer, ASU), and transferred by electroporation to A. tumefaciens EHA101 competent cells.
AFSF and ASSS were then merged to form the final assembly (designated as AFSF + ASSS), as well as the four individual assemblies of AF, SF, AS, and SS (designated as AF + SF + AS + SS).
If φ (defined below) is less than a sensitivity margin σ, then the vector S t is considered stable and its final state designated S, (2) φ (S t ) = 1 τ ∑ i = t - τ t D (S i, S ¯ t ) where D X, Y = ∑ i = 1 N (X i - Y i ) 2 ∕ (4 N ) lies in the interval [0, 1] and S ¯ t is the average of the expression levels over all times from τ- t to t.
The twenty-eighth and final letter designates "the levels, stations, and stages", that is, the invisible degrees of perfection achieved by the unfolding of human souls on the path of the Return.
