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Thereby, the final construct was measured with only three questions.
Peripatellar circumferential cerclage (a); modified anterior tension band (b); final construct (c).
The final construct was introduced into wild-type Dongjin by Agrobacterium-mediated transformation.
The final construct was named as pET scFv apoptin and transformed into competent Top 10F′ cells.
This final construct was transformed into wild-type cells by electrotransformation, as described previously (Han et al. 2015).
Although numerous biomaterials have been investigated as scaffolds for cartilage tissue engineering, the effect of their microstructure on final construct characteristics remains unclear.
The final construct, named Omp34-4, encompasses three copies of L3, two copies of L6 and L7 and one copy of L1, L4 and L5.
The final construct produced 0.88 g L−1 BT from 10 g L−1 d-xylose with a molar yield of 12.82%.
Afterwards, the amplified fragment of SVP2 signal peptide with EcoRI and SacI restriction sites was also cloned into pQE80L-TtL and the final construct pQE-STL was obtained.
The two homologue arms corresponding to each lsp gene were cloned into the appropriate sites of the suicide vector pEX18GM, resulting in the final construct (Table 1).
The final construct was called 35S-mAmFH.
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