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The final consensus base was called when the position had a consensus quality score of at least Q30 and a mapping quality score of at least Q20.
A final decision was made by consensus based on an evaluation of the results of the F-FDG-PET/CT and the characteristics of the conventional workup.
Some indicators were modified or combined due to overlap between categories or pragmatic reasons concerning implementation, resulting in a final consensus-based set of indicators.
Finally, the results from all these procedures are parsed and presented as two web pages: the first one presents all the original results; the second one contains the final consensus-based predictions by integrating the results from different predictors.
Both this PSP and the NIAA's 2009 priority setting exercise employed similar methodology, with an initial research ideas-gathering stage followed by a second prioritisation stage and a final consensus-based stage to agree the foremost research priorities.
The final consensus diagnoses were based on the data collected from the records, the OPCRIT process, and the SCID interview.
STS data were analyzed by at least two independent scientists and the final consensus haplotypes were based on redundant sequence coverage over all positions (at least two independent sequence reads).
The latter analysis showed that that one of the four runs sampled a slightly different posterior distribution than the other three runs, so the final consensus tree was based on the combined posterior distributions of the three similar runs.
The first 2500 trees from each run were discarded so that the final consensus tree was based on the combination of accepted trees from each run (a total of 30,004 trees).
In the final consensus sequence each base matched at least Phred quality score of 35.
A final consensus ranked thematic list was produced based on these median ranks (Step 10, Figure 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com