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Metformin and AICAR were used at final concentrations of 2 and 0.5 mm, respectively.
Final concentrations of purified oligos were quantified using Qubit dsDNA HS Assay Kits (Life Technologies).
7B2-PAC-Alexa488 and sCD4-183 were added to final concentrations of 1 µg/ml each.
4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) was used at final concentrations of 200 or 500 nM.
The final concentrations of monomer and peptide were 100 μg ml−1 and 200 nm, respectively.
GR159897 (Tocris) was used at the final concentrations of 0.04, 0.016 and 0.008 mM in 10% dimethyl sulfoxide.
8 × 104 cells were added to IT to reach the final concentrations of 5 ng/ml to 5 µg/ml.
hPLCζ cRNA was microinjected in the cytoplasm of human eggs final concentrations of 0.05 mg/ml and 0.2 mg/ml.
The initial and final concentrations of MO were determined using a UV visible spectrophotometer.
The final concentrations of 2′-FL and 3-FL were 9.12 and 12.43 g/L, respectively.
Final concentrations of DMSO did not exceed 0.1%.
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CEO of Professional Science Editing for Scientists @ prosciediting.com