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The final concentrated extract was further fractionated using anion exchange chromatography to yield partially purified NaVCP extract.
If the average sample contributed to the pool was 1 mL, and if the final concentrated virus were in 1 mL, the final concentration of a totally new virus would be close to that in the original individual sample.
DHPC concentrations usually varied between 150 and 300 mM in the final concentrated syb/DHPC sample.
The final concentrated COD amounted to 7500 mg/L after 6 days of operation.
The purity of the final concentrated product exceeded 95% when analyzed using Quantity One software (Figure 2G).
The final concentrated samples were stored at −20°C.
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Prior to crystallization, the buffer for native protein was exchanged in the final concentrating step to 10 mM Tris/HCl pH 7.4, 100 mM NaCl, 0.15 % Cymal-5.
For 500 ml cell culture SN the standard UC protocol requires overall 4 runs each lasting 2 hours (3 times at 61,700 g with a loading volume of 180 ml per run and a final concentrating run at 53,500 g).
The buildup to this semi-final concentrated heavily on Lionel Messi and Arjen Robben – and it felt like both coaches' team talks were no different.
The Raglan flowsheet bypasses this first rougher concentrate directly to final concentrate.
A final concentrate containing 23.47% B2O3 with 81.78% B2O3 recovery was obtained from these tests.
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