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A final concentrate containing 23.47% B2O3 with 81.78% B2O3 recovery was obtained from these tests.
The froth phase serves an important role in upgrading the final concentrate in flotation.
The Raglan flowsheet bypasses this first rougher concentrate directly to final concentrate.
After four rougher stages, a final concentrate containing 1.54%Nb2O5with93 93% Nb recovery in ∼15% of the mass was obtained.
A final concentrate of 64.46% Fe, 2.66% Al2O3, and 2.05% SiO2 with Fe recovery of 69.03% in flotation stage and 34.13% with respect to the initial feed was obtained with reverse cationic flotation.
Since UG2 has a significant chromite (FeO·Cr2O3) content, minimising chromium oxide (Cr2O3) recovery into final concentrate has become a critical aspect of downstream smelting since % Cr2O3 in furnace feed has a practical upper limit of about 2.5%.
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The final concentrated extract was further fractionated using anion exchange chromatography to yield partially purified NaVCP extract.
Prior to crystallization, the buffer for native protein was exchanged in the final concentrating step to 10 mM Tris/HCl pH 7.4, 100 mM NaCl, 0.15 % Cymal-5.
For 500 ml cell culture SN the standard UC protocol requires overall 4 runs each lasting 2 hours (3 times at 61,700 g with a loading volume of 180 ml per run and a final concentrating run at 53,500 g).
The concentrated dose of fibrinogen provided by fibrinogen concentrate (final concentration 10 times higher than FFP) might be preferable to FFP for restoring plasma fibrinogen levels because of its rapid availability (no thawing), reduced volume (faster infusion time) and increased safety, and it has recently been suggested to be more effective than administration of FFP [ 15].
This fraction was immediately neutralized by the addition of Tris-HCl pH 6.8 (250 mM final concentration), and then concentrated by precipitation with 80% ammonium sulfate.
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