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The fabrication process from exfoliation to final cavity for the fabrication route via EBL-RIE-EBIE is schematically depicted in Supplementary Note 2. First, high crystalline hBN flakes were mechanically exfoliated from sticky tape onto trenched silicon substrates.
Lopez, who was detained for two years and four months on a murder charge before he was acquitted after DNA evidence was presented at a retrial, was also forced to undergo a final cavity search as he was processed for release.
The accuracy of our final cavity detection is evaluated in the next section.
Before performing the final cavity detection, 160 candidate contours are divided into cavity and non-cavity contours.
Three contour-based features (circularity, GICOV, and KLD) are extracted from the candidate contours for the final cavity classification.
Three types of contour-based features, shape, edge and region, are extracted for the final cavity detection.
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We made a final cavity-empty set from the two top quintiles and a final cavity-occupied set from the two bottom quintiles, each with 356 subtomograms.
Then we applied a mask to the difference map between the final cavity-empty average and the final cavity-occupied average that would include the region of the cavity only.
First, we applied a mask to our final cavity-empty average that included the TRiC density only, thus excluding the cavity.
DOI: http://dx.doi.org/10.7554/eLife.00710.007 The final cavity-empty TRiC average from the combined dataset highly resembles apo-TRiC and was produced using the same bias-free approach applied to fibril-bound TRiC ('Materials and methods' under 'Single particle tomography [SPT]').
The mould making process for rotational (roto-) casting products involves manufacturing operations ranging from transforming customers' specification into artistic and functional design, right through to final mould cavity polishing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com