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Microspheres were isolated from the mixture by filtration through a 0.45-µm PTFE filter (4555, Pall Corporation, Russia).
Culture supernatants containing the autoinducer were collected and sterilized by filtration through 0.22-μm pore-sized filters.
Incubation was terminated by immediate filtration through Whatman GF/B glass filters (presoaked with 1 mM pyrocatechol for 3 h).
Any additional cell debris was removed from the medium by filtration through 0.22-μm PES membrane filters (Thermo Fisher Scientific).
After homogenization, the extracted materials were obtained by filtration through filter paper (Whatman no. 1).
First, ZnS was separated from the sample by vacuum filtration through a 0.2 μm filter.
The extracts were separated from the material residue by filtration through Whatman No. 1 filter paper.
The equilibrium concentration was determined using centrifugation and filtration, through a Millipore filter of the suspension.
The nanoparticles are then purified by tangential filtration through 5 kDa membrane.
Filtration through a 0.22 μm filter yielded the final solution, ready for injection.
After filtration through a glass wool filter, the samples were placed in NMR tubes for analysis.
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