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RM, SM and Golgi fractions were prepared for electron microscopy using the random sampling filtration protocol detailed previously (Bell et al., 2001).
A differential centrifugation and filtration protocol was developed to enrich for viral material.
Virus was concentrated by using an adsorption-elution-ultrafiltration filtration protocol (4 ).
Transport assays were performed based on the rapid filtration protocol established by Leier et al.
We used a filtration protocol reported previously [ 19], which generated R and HSW samples from each urine sample.
We next examined those regions of the genome that performed poorly in the whole genome capture technique as compared to the leukocyte filtration protocol.
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As the Cot filtration protocols were described in detail in those two references, we describe briefly the overall approach related to the cattle tick.
Alkaline pretreatment of A-beta42 and preparation of low molecular weight A-beta by filtration protocols were used before beginning the globulomer preparation.
Filtration and washing protocols for supported reagents were performed in a 12-channel vacuum manifold.
They included a filtration step in their protocol, aimed at isolating purely the microvesicle component of the serum, and treated the resulting filtrate with RNase to remove non-vesicle associated RNA.
The procedures used as a reference protocol were filtration of 10 mL water samples through sterile syringe filters with 0.22 μm pore size (PES, Millipore, USA) followed by fixation with glutaraldehyde (0.25% final concentration) for 15 min at 4°C and freezing in liquid nitrogen during transport and storage at −80°C after arrival in the laboratory.
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