Sentence examples for filtration plate from inspiring English sources
The proteins were eluted stepwise in a high concentration using EDTA by centrifugation, while the resin in the filtration plate was washed on the vacuum manifold.
The consecutive process, from the dialysis cell-free protein synthesis to the partial purification by immobilized metal affinity chromatography on a 96-well filtration plate, was performed within ca. 14 h, including 8 h of cell-free protein synthesis.
Total DNA was extracted from ethanol-stored fin-clips with either a silica-fines/microtitre filtration plate method [33], or phenol-chloroform [34].
Assays were set up using either 3.5×105 undepleted or CD25hi-depleted cells in RPMI 1640 supplemented with 5% heat-treated FCS, 100 U/ml penicillin, 100 µg/ml streptomycin, 2 mM L-glutamine and 1 mM sodium pyruvate per well of a polymer-backed 96-well filtration plate MAIP-S-4510 MAIP-S-4510 MAIP-S-4510 France) in a total reaction voluMillipore µl/well.
Reaction mixtures were then processed using a filtration plate (Millipore MultiScreen Barex/TiO2 plates) as previously described [ 31].
Assays were performed in a 96-well filtration plate (Millipore) with 5,000 beads coated with human IL-8 antibody.
Multiscreen 96-well immobilon-P filtration plates were coated with purified anti-IFNγ (4 µg/ml; R&D) overnight at 4°C.
Freshly isolated bone marrow and spleen cells (1×106 cells) were added to the Multiscreen 96-well filtration plates (Millipore) coated with inactivated rgΔH5N1 virus antigen.
96-well Multi-screen HA Nitrocellulose filtration plates (Millipore) were coated with 50 µl of 10 µg/ml recombinant MSP1 diluted in PBS.
Cells were harvested and transferred to 96-well Multi-screen HA Nitrocellulose filtration plates and an ex-vivo ELISpot assay for MSP1-specific plasma cell detection performed as described above.
Briefly, multiscreen 96-well filtration plates were coated with IL-4 and IFN-γ capture antibodies at 4°C overnight and were blocked with 10% FBS in RPMI for 2 hr at 37°C.
