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gel filtration column.
One more filtration column was made with ordinary sand alone without Moringa extract.
Conjugates were then purified using a Sephadex G-50 (1 × 20 cm) gel filtration column.
33 was further concentrated and loaded on Biogel P-150 gel filtration column.
A filtration column was made using a container with perforations at the bottom.
The mixture was loaded onto a gel filtration column (Superose6 10/300 GL, GE Healthcare).
Gel filtration column chromatography indicated that subunit II formed multimeric complexes with four or more subunits.
These fractions were pooled and loaded onto a Superdex 75 gel filtration column.
The protein was filtered again and injected onto the Superdex gel filtration column for buffer exchange.
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