In addition to stemness genes, we also filtrated out 15 differentiation-related genes from in yHCCs.
It should be noted in the Materials and methods how the excess of His6-bZIP63 was filtrated out during the mass spectrometry analysis.
Initially, genes were filtrated out for obvious annotation errors, such as coding sequences that were not a multiple of three nucleotides.
Next, the dreg was boiled once again for 1 h with 2 L of water, and the decoction was filtrated out with the above method.
The PAGE-zymogram analysis of the concentrated culture filtrate, carried out on guaiacol, confirmed the expression of laccase in active form.
Then 5 ml of the filtrate was pippetted out into a test tube and mixed with 2 ml (10 fold diluted) of 0.1 M FeCl3 in 0.1 N HCl and 0.008 M potassium ferrocyanide.
Treatments with fungal culture filtrates were carried out 10 min after dye loading and extensive washing.
On the other hand, the ethanol in the filtrate solution was distilled out at 80°C, and the surfactant was recrystallized in a mixture solution of acetone/ethanol (95:5 in volume) after the acid in the solution was neutralized[20].
In fruitflies and other insects, ultrafiltration and filtrate modifications are carried out in separate cell types and organs (ultrafiltration: nephrocytes; filtrate modification: Malpighian tubules) (Dow and Romero, 2010).
The process was carried out by filtration and both the filtrate (solid phase) and the liquid phase were analysed by gas chromatography (GC), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA).
