Bioinformatic analysis followed previously described protocols, including normalization, filtering, assignments of functional ontogeny, and gene set enrichment for pathway identification [ 40].
You can easily filter assignments relevant to you by narrowing down the province and city and the client.
These two assumptions are translated into the EXPANDS model in four main steps: cell frequency estimation, clustering, filtering and assignment of mutations to clusters (Fig. 1).
After filtering, COG assignments of the putative gene products were generated according to COG identification, using the best-hit pair in the reference data set.
After filtering, COG assignments of the putative gene products were generated according to COG identification, taking the best-hit pair in the reference data set.
Results of the taxonomic assignment were filtered to retain assignments with >10 sequences.
Since most of the assignments are inconsistent, ARIA performs an iterative protocol to identify wrong assignments and noise peaks: at each iteration, the restraint list is corrected by filtering out unlikely assignments and noise peak, and then, based on the filtered restraint list, a new structure ensemble is calculated.
Additional file 1: The table of OTUs for all the samples at the genus level with OTU assignment before filtering process.
(XLS 81 kb) Additional file 2: The table of OTUs for all the samples at the genus level with OTU assignment after filtering process.
Using a combination of probability assignments and filtering based upon peptide pI, as we have previously described [24], we obtained a high confidence catalogue of 578 proteins (false positive rate <1%).
