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The time-evolving states characterizing the neuron dynamics, including a filtered membrane potential and the dynamics of the gating variables 2.
Supernatants were harvested 48 h post-transfection and filtered (membrane pore size = 0.45 μm).
Spikes were detected as events where the membrane potential deviates >+30 mV from the high-pass filtered membrane potential (>400Hz).
We then sorted the filtered membrane genes according to their expression levels in hESCs (Supplementary information, Table S1).
To detect Coli-like, P. aeruginosa and faecal enterococci, 3 × 100 ml of water were filtered (membrane filter with pore size of 0.45 μm; Schleicher & Schüll, Dassel, Germany).
The filtered membrane potential data were then grouped into similar voltage ranges to ensure that average membrane potential did not differ between hit and catch conditions.
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The samples were filtered through membrane filter (0.45 μm) using syringe filter.
Aqueous phase was filtered through membrane filter with a pore size of 0.45 μm.
Before injection, all samples were filtered through membrane filters with 0.45 μm pore size.
The incubation water containing Chaoborus kairomone was filtered through membrane filters (pore size: 0.45 mm).
Before use, brines were filtered by a 0.45 μm2 filter membrane.
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