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Raw data from each hybridization was normalized by Robust Multichip Average (RMA), background corrected, and filtered for presentation using GeneSifter software (Geospiza Inc., Seattle WA).
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Recordings were low-pass Bessel filtered at 10 kHz by the amplifier and smoothed with a 1 kHz Gaussian filter for presentation.
Results were filtered for bi-directional hits.
Journalists can filter for their interests.
You want to filter for lead.
Background was subtracted and in some cases a median filter was applied only for presentation.
Single pixel shot noise was reduced for presentation by trend filtering each pixel along the time axis (Methods).
The actual values will not be presented at any time (except for values specified for presentation), but they allow for finer data filtering during querying.
The background of each channel (mean of empty region) was subtracted and in some cases a median filter was applied (radius: 1 pixel) only for presentation.
Shot noise was reduced for presentation by sequentially applying a trend filter to each spatial and temporal dimension (Methods).
For presentation to the radiologist for reporting, consideration should be given to applying a post-filter, but no post-filter should be applied when using the image for quantification purposes.
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