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The mixture was filtered with a 0.22 µm Millipore nitrocellulose membrane filter under vacuum conditions and it was rinsed with fresh n-pentane until the eluent became colorless.
The mobile phase consisting of acetonitrile-30 mM phosphate buffer adjusted to pH 3.5 with orthophosphoric acid (30 70, v/v) was filtered through a 0.45 μm Millipore membrane filter under vacuum.
Cell homogenates were cleared by centrifugation, filtered through a 1.2 µm prefilter and then through a 0.45 µm filter under vacuum.
The supernatant was filtered using 0.45 μ filter under vacuum.
The extracts, vincaria (RN180) or RNI 249, and their combination (Reparagen™) were supplied by Rainforest Nutritionals, Inc and were dissolved in water and filtered through a 0.45 μm filter under vacuum prior to use.
This was then filtered through a Whatman 934-AH microfibre glass filter under vacuum and 48 ml of the filtrate applied to an IAC containing antibodies specific for OTA (VICAM OchraTest™).
Similar(53)
The homogenates were filtered through filter paper under vacuum, and the soybean hydroalcoholic extract (HE) was obtained.
The solution was filtered under vacuum.
Suspensions were filtered onto sintered filters under vacuum.
After cooling, the extracted solutions were filtered under vacuum.
The contents of the flasks were filtered under vacuum through a Whatman #4 filter paper.
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