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Following cigarette filter preparation, storage conditions were kept constant for each cigarette examined in this work until smoking.
Besides this, it shall be outlined the filter preparation tool for terminology extraction which has been especially designed for this work as well as the corpus representativeness measurement method employed.
In general, the methods used in this study, including, sequence collection, BAC high-density filter preparation, Overgo hybridization, and anchoring the BAC contigs to individual chromosomes, are reliable methods.
FS supported the BAC library assembly and filter preparation.
Information about sampling procedure, filter preparation, extraction of nucleic acids, sequencing strategy, sequence quality check and taxonomic assignment (on the phylum level) of the samples can be found in Logares et al. (2014) [ 18].
This was facilitated by the robotic picking of a large number of clones (over 18 thousand) and the automated library duplication and filter preparation features of the Q-bot.
Similar(54)
The supernatant was filtered through a 0.2 µm filter and the filtered preparation was used for infection of flies.
The resulting amyloid fibrils were collected via a reverse centrifugation after resuspending the filtered preparation with the same initial volume of 20 mM Mes, pH 6.5.
The first round was to screen BACs libraries using Overgo probes (44 base pair long) and protocol for BAC-filters preparation, Overgo probe design, pre-hybridization, and hybridization were same as [50].
Following resuspension of the filtered preparations with the filtrate (20 mM Mes) to reach the initial concentration, the resulting products were collected via a reverse centrifugation.
Furthermore, a more detailed investigation on the EOP estimation is presented in (Karbon et al.: EOP estimated from VLBI with a Kalman filter, in preparation).
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