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TSA containing 5% defibrinated sheep's blood plates using both spread and filter plating technique were used for Enterococcus faecalis, Streptococcus pneumoniae and Streptococcus pyogenes.
TSA containing 5% defibrinated rabbit's blood plates using both spread and filter plating technique were used for Listeria monocytogenes, ATCC 7644.
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Skip to instruction Step 14. Transfer samples to Millipore Filter plate (MSNU 03050).
A commercially available 96-well filter plate (0.3 cm2 per well) and a custom designed 8 24-well filter plate (0.8 24-well well) were used to quantitatively study the microfiltertion characteristics of an Escherichia coli TOplateermentation broth.
Fig. 1 Filter plate of the self-made reactor used in this study.
The 96-well plates (MulitScreen Filter Plates, HTS) were washed with 250 μL 10 mM TRIS buffered saline (pH 7.3).
Bound and free radioligand were separated by vacuum filtration through glass fiber 96-well filter plates (Millipore Multiscreen FB filter plate), followed by washes using ice-cold assay buffer (3 × 150 μL).
The filter plates were then dried and counted by standard liquid scintillation counting techniques in a TopCounter (Perkin-Elmer).
Blood samples were filtered using the multi-well filter plates, and the DNA was extracted.
Premixed anti-cytokine biotinylated antibody capture beads were dispensed in a pre-moistened filter plate.
The system allows simultaneous identification of cytokines in a 96 well filter plate.
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