Fig. 1 Filter plate of the self-made reactor used in this study.
A commercially available 96-well filter plate (0.3 cm2 per well) and a custom designed 8 24-well filter plate (0.8 24-well well) were used to quantitatively study the microfiltertion characteristics of an Escherichia coli TOplateermentation broth.
Premixed anti-cytokine biotinylated antibody capture beads were dispensed in a pre-moistened filter plate.
The system allows simultaneous identification of cytokines in a 96 well filter plate.
Amplicons were purified using MultiScreen PCRµ96 Filter Plate (Millipore, Billerica, MA).
Following pre-wetting of the filter plate, 50µl of bead suspension was added to each well and washed twice.
PCR products were purified using the multi-screen PCR filter plate (Millipore, Saint-Quentin en Yvelines, France).
In brief, the appropriate cytokine standards and samples diluted in plasma diluents were added to a 96 well filter plate.
In short, the MultiScreen HTS™ 96-well filter plate (Millipore, Inc., Massachusetts, USA) was pre-wetted with assay diluent for 5 min, 100 µL/well.
In vivo GFP imaging was visualized and measured by an illuminating device LT-95000 IllumaTLSl TLS equipped with excitation illuminating source (470 nm) and filter plate (515 nm)).
DNA was extracted and purified following the protocol of Ivanova et al. [29], using a 96-well filter plate with 1 ml wells and 3.0 µm glass fiber.
