0.5 g of each sample was suspended in 4.5 ml of filtered (0.2 µm pore-size filter) ice-cold phosphate-buffer-saline (PBS) and vortexed with a dozen of glass beads for at least 3 min to homogenize the sample and dislodge the bacteria from feed particles.
Ninety thousand litres of pure water will be made at the Ellsworth site by heating and then filtering ice, using a boiler taken along for the purpose.
The machines use the cold drip method, a technique that doesn't use hot water or electricity, but instead crafts coffee with small round filters, ice water and gravity, the cheapest form of energy.
Embryonic day 11.5 (embryosembryos were dissected from the uterus and washed several times with filtered, ice cold PBS.
Frozen tissue sections were first fixed in filtered, ice-cold acetone and next in 4% paraformaldehyde.
The IgG-Sepharose was rapidly and thoroughly washed on a scintered glass filter with ice-cold PBS- 5 mM EDTA and bound proteins were eluted with ice-cold CAPS buffer (50 mM CAPS, 1 M NaCl, 5 mM EDTA, pH 11.0 to 11.2).
Bacteria were resuspended from the filter with ice cold nuclease free water.
defer.add img); Strong tea defer.add img); When making iced tea, hot tea can be filtered using ice cubes.
Finally, spermatozoa were obtained by gently filtering on ice through fine nylon mesh to remove tissue debris.
The reaction was allowed to proceed for 10 min at room temperature, following which the free nucleotide was removed from bound by applying the mix to 0.45 μm nitrocellulose filters in duplicate in a Brandel cell harvester attached to a vacuum pump and washing the filter with ice-cold wash buffer.
Lavage fluids were filtered through sterile gauze filters, collected on ice, and immediately centrifuged at 1,500 g for 10 minutes.
