After centrifugation, the supernatant was collected, sterile filtered, dialyzed against highly purified water, filtered, frozen, and lyophilised.
The seawater samples were filtered through GF/F 0.7 μm glass filters, frozen, and stored in the freezer.
For fatty acid analysis, 100 mL of the culture were filtered through pre-combusted GF/F (25 mm) filters, frozen in liquid nitrogen and stored at −80°C until further analysis using gas chromatography (GC) and gas chromatography mass spectrometry (GC-MS).
CM were filtered, frozen, and analyzed on antibody arrays (Chemicon; Mouse cat #AA1003M-8) as recommended, with previously described modifications [15].
The infusion was filtered, frozen in a freezer, and then lyophilized.
The solution was filtered, frozen and then lyophilized to obtain the aqueous extract, with a recovery rate of 17%%.
The pH was again adjusted to physiological conditions, and the extract was sterile filtered, frozen, and lyophilised.
Then, supernatants were recovered, filtered, frozen at −20 ° C until use for in vitro and in vivo experiments (see below).
Virus supernatant was collected 48 h posttransfection, filtered through 0.45 µm filters, frozen in small aliquots on dry ice, and stored at −80°C.
The mycelia of the CM and MM plus AVICEL cultures were filtered, frozen in liquid nitrogen and freeze dried prior to the determination of dry weight and total protein content, respectively.
