Sequence reads were simply filtered for unique 64 base sequence reads that were present in five or more lines and these were mapped genetically as described below.
These sequences were mapped back to the human reference genome using BWA and filtered for unique mappings.
The complete set of STS markers was filtered for unique and unambiguous marker-assembly sequence alignments as described.
Manually filtering for unique strands with three or more hot spot residues resulted in a set of 172 entries describing 133 distinct protein protein interactions.
To eliminate duplicate or obsolete SNPs, the LD80 SNPs were further filtered for unique genomic coordinates in hg19 through BioMart, and analyses were conducted on these LD80 SNP sets.
Building on the Bio-SamTools distribution, ViennaNGS::Bam provides a set of convenience routines for rapid handling of BAM files, including filters for unique and multiple alignments as well as functionality for splitting BAM files by strand, thereby creating two strand-specific BAM files.
Within minutes I had built an application (also known as a pipe, they should probably change the name as not everything can be a pipe) that would search for 'Techcrunch' in a variety of feeds, bring that data together, sort it and filter it for unique results.
Introducing the permutation test and the filter for non-unique matches improves the reliability of the results (Experiment 2).
As can be seen from Experiment 1, without the permutation test or a filter for non-unique matches, the best matches have numerous overlaps that have no sequence support, suggesting that they are poorly placed.
To create MDACC-701, we downloaded a total of 1,128 tumor profiles from the five datasets then filtered for the unique (nonredundant) profiles using a custom script to measure correlations between all pair-wise combinations.
