Animals were exposed to odors by placing a filter paper dish (70 mm diameter, #1440 070; Whatman, Florham Park, NJ, USA), impregnated with 0.4% odorant in 10 µl of odorless mineral oil (odorants and mineral oil obtained from Sigma) in the lower compartment.
Subsequently, the retina was transferred to a Millicell culture dish filter insert (Millipore AB, Solna, Sweden; PIHA03050) with the retinal pigment epithelium layer facing the membrane.
Explanted retinae were cultured on Millicell HA culture dish filter inserts (Millipore, Carrigtwohill, Cork, Ireland; PIHA03050) with the retinal pigment epithelium facing the membrane.
In brief, the retina and the retinal pigment epithelium (RPE) were isolated and subsequently transferred to a Millicell culture dish filter insert (Millipore AB, Solna, Sweden; PIHA03050), with the RPE layer facing the culturing membrane and incubated in R16 nutrient medium at 37 °C.
Surface sterilised seeds were germinated by placing them on sterile wet filter paper in dishes.
Seven days before the bombardment, embryogenic calli were dispersed over a 0.45 μm Membrane filter in Petri dishes containing C medium with 10 μM 2,4-D (C10 medium).
In these shaping sessions the filter paper in one dish contained the EB stock (S+) and the other dish contained filter paper with IA stock (S-).
The germinated seeds were transferred into Petri dishes with filter paper and cultured in water (25 seedlings per dish, one biological replicate), and five independent biological replicates were employed, with two for sequencing and the other three for experimental verification.
During the initial shaping trials one dish contained filter paper with five μl of mineral oil adsorbed onto it, while the other dish contained filter paper with five μl of EB stock odor.
