These slices were transferred to a 0.40-µm filter cup (Millicell-CM, Millipore, Bedford, MA, USA), placed in a standard 6-well plate, and cultured with 1 mL of medium that consisted of 50% Eagle's basal medium, 25% Earle's balanced salt solution, and 25% heat-inactivated horse serum, supplemented with 5 mg/mL glucose and 1∶100 Glutamax (Invitrogen, Carlsbad, CA, USA).
Hydrolysate was then sterile filtered through a 0.22-um filter cup.
The remaining sample in a filter cup, the retentate (R), was mixed with the same volume of 2x solubilizing buffer (2x Laemmli buffer with 400 mM dithiothreitol).
Venus fluorescence was observed on the culture plate using the Lighttools Illuminatool Tunable Lighting System equipped with a 535 nm viewing filter and 488 nm/10 nm filter cup.
To observe the gross nodule of liver metastasis, the whole livers were imaged under a fiber optic illumination LT-9900 Illumatool Bright Light System (Lightools Research, Encinitas, CA), and imaging was carried out at 470 nm with LT-9470FX 470 nm in Lightools Filter Cup (Lightools Research, Encinitas, CA).
We transferred monolayers of A6 cells subcultured on tissue culture-treated Transwell filter cups to a modified Ussing chamber (Jim's Instrument, Iowa City, IA, USA) designed to hold the filter cup and continuously measured transepithelial potential difference (PD) by a high-impedance millivoltmeter (VCC-600, Physiologic Instrument, San Diego, CA, USA) [ 7].
DNA was purified from the gel slurry by passing the samples through Sample Filter Cups from the DNA Gel Extraction Kit (Millipore, Billerica, MA, USA) by centrifugation at 4965×g for 10 min at room temperature.
Then, we seeded cells onto permeable tissue culture-treated Transwell filter cups (Costar, Cambridge, MA, USA) for electrophysiological measurements at a density of 5 × 10 cells/well for 11 15 days.
The new catalog of HARNEY & SONS in Salisbury features dozens of teas and tea accessories (pots, filters, cups, scoops, mugs).
"Lotta wildlife on the road?" David asked, fussing with filters, cups, potholders, avoiding Danielle's curious expression.
Measure out three-fourths of a cup of coffee grounds and dump them into the filter-cup.
