The term "operational amplifier" describes an important amplifier circuit that can form the basis of audio and video amplifiers, filters, buffers, line drivers, instrumentation amplifiers, comparators, oscillators, and many other analog circuits.
And dry-land farmers have developed many strategies to collect rain via barrels and cisterns for direct use, and to resupply groundwater using bioswales (land features that filter water), buffer strips, off-stream storage reservoirs and natural wetlands.
The agriculture runoff which directly enters the river can be reduced by constructing filter and buffer strips around agricultural fields adjacent to the riverbank.
The reaction was terminated by addition of 3 ml of ice-cold filter wash buffer and rapidly vacuum filtered through glass fiber filters (Whatman GF/C).
Importantly, our results showed that this acute adverse effect of PEEP was effectively negated by nonassociative learning acting as a high-pass filter or buffer for the tonic input.
The eluent was concentrated with an Amicon Ultra centrifugal filter, and buffer exchanged on a PD-10 desalting column.
The filter paper, buffer, and inhibitor mixture were equilibrated at 50 °C for 5 min in a reciprocating water bath agitated at 100 rpm.
However, the PEG precipitation resulted in an extremely viscous solution that could not pass through the centrifugal filter during buffer exchange.
"In the filter-binding assay, a 5'SS RNA oligonucleotide (5'-P-CAAAGGUAAGAUGGA-3', 10 fmol), was mixed with various concentrations of U1 snRNP, with or without U1-C protein, in Filter Binding Buffer (FBB: 200mM KCl, 2 mM MgCl2, 0.5 mM DTT,µg/mL10tRNA,mM Hepes, pH 7.5, 100 50µg/mL BSA) in a final volume of 40 µl and incubated at 22°C for 2 hours.
