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The optical immuno assay (OIA®) thin film detection technology utilizes an optically coated silicon surface to convert specific molecular binding events into visual color changes by altering the reflective properties of light through molecular thin films.
MSAP requires the use of a radiolabeled substrate, which can be harmful to human health, and its reaction products need to be analyzed by gel electrophoresis followed by X-ray film detection.
Immunoreactivity was detected by incubating each membrane with horseradish peroxidase-conjugated donkey anti-mouse or anti-rabbit (1 1000; Amersham, UK) followed by enhanced chemiluminescence (ECL; Amersham Biosciences) and film detection (Amersham Bio-Max).
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Generated blots were exposed to Roche® Lumi-film Chemiluminescent Detection Film for 3 to 24 hours prior to development.
and the membranes were exposed to Lumi-Film Chemiluminescent Detection Film (Roche Applied Science).
Protein bands were visualized using Chemiluminiscence blotting substrate-POD (Roche) on Lumi-Film-Chemiluminiscent Detection Film (Roche).
The blots were exposed for 25 min up to 3 h to a Lumi-Film Chemiluminescent Detection Film (Roche Applied Science).
DIG Easy Hyb, Anti-digoxigenin-AP, CDP-Star, positively charged nylon membrane and Lumi-film chemiluminescent detection film were used (Roche Applied Science, Indianapolis, IN) as well as the Qiagen Quick-change II XL kit (Qiagen Inc., Valencia, CA).
With the molecularly imprinted polymer (MIP) film, specific detection of bilirubin was successfully achieved.
The electrocatalytic properties of the PB film enable detection of an enzymatic by-product (H2O2) at a very low applied potential: 0.0 V against SCE.
MIP film for detection of caffeic acid has been developed on QCM electrode and selectivity experiments and analytical performance of caffeic acid imprinted QCM nanosensor has been studied.
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