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Pickup jam sessions are held every Wednesday night in the Jamhouse's insulated basement, which the students filled with recording equipment; student bands can also use the space to rehearse.
Horizontal slices (400 μm) were collected on a vibratome and transferred to a chamber filled with recording ACSF (in mM: NaCl, 124; KCl, 3.5; NaH2PO4, 1.25; MgCl2, 1.5; CaCl2, 1.5; NaHCO30 30; glucose, 10), continuously bubbled with 95% O2 and 5% CO2.
Pipettes (BF-150-86 borosilicate glass, Sutter Instruments, Novato CA) were pulled on a P-87 microelectrode puller (Sutter Instruments) and fire-polished with an MF-830 microforge (Narishige, Japan) to a resistance of 1 4 MΩ when filled with recording solution (in mM: 108 CsCH3SO3, 4 MgCl2, 9 EGTA, 9 HEPES pH 7.20 with CsOH).
For whole-cell recording, the recording pipette had a 2 4 MΩ resistance when filled with recording solution (131 mM K-gluconate, 10 mM HEPES, 5.5 mM EGTA, 1 mM CaCl2, 2 mM MgCl2, 3 mM NaCl 5 mM KCl, 0.1 mM ATP, and 0.1 mM GTP).
Stimulation of Schaffer collaterals from the CA3 region was performed with bipolar electrodes, while borosilicate glass capillary pipettes filled with recording ACSF (resistances of 2 3.5 MΩ) were used to record field excitatory postsynaptic potentials (fEPSPs) in the CA1 region.
Hippocampal slices were incubated for 1 h at room temperature and then transferred to a heated recording chamber filled with recording ACSF (125 mM NaCl, 2.5 mM KCl, 1.25 mM NaH2PO4, 25 mM NaHCO3, 1 mM MgCl2, 2 mM CaCl2, and 10 mM glucose, saturated with 95% O2 and 5% CO2) maintained at 32°C.
Similar(54)
To add to the atmosphere, the spaces are filled with recorded roaring.
If 511 is after, 531 is before, with long corridors lined with metal cages filled with records.
Roman history is filled with records of victories and triumphs over Etruscan cities, especially in the south.
The lows and woes continued for network series on Thursday night, extending what has been a week filled with record-setting depths for a raft of shows.
"Derek said, 'Remember that you're the best.' " In a career filled with record-breaking performances and memorable occasions, Rivera said, his favorite moment was winning the 2009 World Series against the Philadelphia Phillies.
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