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We filled the solution with distilled water to the initial volume every 12h after treatment.
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The reference electrode was immersed into a small bath filled with the solution for Cu deposition.
Both the Luggin capillary and polymer tube were filled with the solution for Cu deposition.
The sample is initially filled with the solution at Cin (t0), t0 being the instant when the experiment starts.
In one group of experiments, the cell filled with the solution was initially adjusted at the hydrate solution equilibrium temperature, Teq(4.4°C).
Patch pipettes were filled with the solution mentioned in the preceding section.
Small glass wells were filled with the solution and covered with a coverslip.
For this, a 1 mL syringe filled with the solution of fMLP and fibronectin was connected to one port of the device, and the outlet port was blocked.
In the next step, the OTS nanopores were filled with the solution of precursor salt.
The cell was then filled with the solution containing protein and sealed.
Before weighting, the bottle containing the sample was placed into an ultrasonic bath for 10 minutes to ensure that every pore was filled with the solution.
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