Sentence examples for fill vibratome from inspiring English sources
Brains were fixed in 4% PFA overnight and cut sagittally with a vibratome the next day.
Tissue sections were cut on a Leica VT-1000S vibratome.
Vibratome at speed ~1, amplitude 8 (approximate and will vary).
Following electroporation, brains were dissected and vibratome sectioned at 250 μm.
70-µm thick slices were vibratome sectioned in PBS (0.01 M, pH 7.4).
The pre-chilled chamber of the vibratome is filled with ice-cold oxygenated aECF just before use and is then placed in the ice-filled tray of the vibratome.
The Vibratome bath was then filled with ice-cold sterile phosphate buffer saline- containing 100mg/ml heparin (PBS-H) (we added heparin as an inhibitor of RNase for subsequent procedures not described here).
Retrogradely labelled neurons were only included in the sample if their nucleus (identified as a filling defect) was entirely contained within the Vibratome section, or if part of the nucleus was present in the first optical section in the z-series (corresponding to the top of the Vibratome section).
A block of tissue containing the midbrain was placed in a Vibratome (Coretech, St . Louis MO, USA) filled with the same solution and cut in horizontal slices (thickness: 350 μm).
The brain tissue was mounted onto a block and transferred to the sectioning stage of a Vibratome DTK-10000, DSKyotooto, Japan) filled with ice-cold ACSF such that the cutting stage and blade were completely submerged.
