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To help fill remaining gaps, the CDC and Wiedmann often collaborate.
Because mixed stands are projected to dominate future forests, it is essential to fill remaining gaps in knowledge about the effect of mixing on tree structure and wood quality (i.e., branchiness, specific density of wood, defects) (Pretzsch and Rais 2016).
In the fifth step, LA-PCRs were performed to fill remaining gaps between scaffolds using TaKaRa LA Taq™ (TaKaRa, Shiga, Japan).
We are confident in our findings, having routinely sequenced whole bacterial genomes [ 35- 37] and mapped the Akkermansia muciniphila reference genome to fill remaining gaps.
Standard ancient DNA singleplex PCRs, cloning and Sanger sequencing approaches were used to fill remaining gaps and resolve ambiguous sequences after the first assembly of the total reads generated with the 454 technology.
Estimating the cost of the activities required, accounting for what resources are already available, and identifying how funding could be provided and by whom to fill remaining gaps would be essential to better inform the diverse set of partners who may be able to contribute to these efforts.
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Gaps resulting from repetitive sequences were resolved by in silico gap filling, remaining gaps were closed by PCR followed by Sanger sequencing or by long reads from a Pacific BioSciences sequencing run (PacBio RS, 150,305 reads, 174.8 Mbps, 38-fold coverage, GATC, Konstanz, Germany), yielding a draft genome of 4,648,936 bps.
They follow up over the phone with those who do not respond and use health-department and other data to fill any remaining gaps.
After all these decisions have been made, the administrators of the various state colleges and universities are left to fill the remaining gaps by increasing tuition or cutting costs.
Governments serious about engaging diasporas have to commit to funding their efforts as fully as possible (and seeking funds from elsewhere to fill in remaining gaps) and to obtaining the operational knowledge and skills needed to pursue goals effectively.
(In early March, the White House extended that deadline to 2016). The administration in December also announced a new catastrophic exemption to fill any remaining gaps in coverage — estimated to affect as many as 500,000 people.
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CEO of Professional Science Editing for Scientists @ prosciediting.com