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The 50.8 × 38.1 × 15.75 mm3 ML-OHP consisted of four inter-connected layers of circular mini-channels, as well an integrated, hermetic-grade fill port.
A flexible liquid helium (LHe) return line on the recycler is inserted into the fill port of the MEG system in the MSR through a slotted opening in the ceiling.
52.5 μl TaqMan Gene Expression Master Mix was added to the cDNA sample before loading 100 μl to each LDA fill port.
Cy3-labeled samples were resuspended in the recommended amount of hybridization buffer and denatured at 95°C for 5 min. Slides were placed in HX12 NimbleGen Mixer and 6 μl of sample loaded though the fill port.
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On both isles it is now the prone bodies of newcomers, many huddled in foetal positions in flimsy, low-lying tents, that fill ports, streets and shores.
100uL of the reaction mix was added to each of eight fill ports on the TaqMan MicroRNA Array.
The filled Array was centrifuged twice at 1200 rpm for one min, and then sealed with the eight fill ports removed.
50 μl cDNA (equivalent to about 0.25 μg RNA) was mixed with 50 μl 2 × RT−PCR master mix and pipetted into two of eight fill ports of the LDA.
The ring is connected to a filling port by a thin tube, so the band can be tightened or loosened by adding or reducing the fluid in the port.
Certain tissue expanders have a magnet filling port and are not MRI compatible.
A short distance from the foundering ship, Baalham's boat encountered the partly filled port quarter boat.
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