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The resultant files were analysed using the QIIME (Quantitative Insights Into Microbial Ecology)54 software package (http://www.qiime.org/) and USEARCH10 19.
GC-MS data files were analysed using MSD Enhanced ChemStation software (Agilent Technologies) to determine sterol profiles for all isolates and for derivation of integrated peak areas.
Raw data files were analysed using the Bioconductor [45] Affymetrix package (http://www.bioconductor.org).
CEL files were analysed using dChip software version 2007 [ 32].
Peaks in the resulting trace files were analysed using the formula stated above.
The FASTQ files were analysed using the open source GAPSS_B v2) pipeline http://www.lgtc.nl\GAPSS.
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The resultant wave file was analysed using BatSound Pro software (version 3.20; Pettersson Elektronik AB, Uppsala, Sweden).
Quality of each raw sequence data file was analysed using open-source software FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/).ac.uk/projects/fastqc/
The EEG file was analysed using Brainvision Analyzer and was corrected for horizontal and vertical (blinks) eye movements using the Gratton and Coles method [ 42].
The results file was analysed using a bespoke Perl program to create an "architecture string" for each sequence, which lists all the domain names in sequence order.
The peak position data from [ 33] were converted from NCBI36/hg18 to NCBI37/hg19 using http://genome.ucsc.edu/cgi-bin/hgLiftOver and the resulting bed file was analysed using GREAT and the UCSC (http://genome-euro.ucsc.edu) browser.
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