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Ms. Castle's personal popularity with the party's rank and file was confirmed in 1950 when she was elected to its national executive committee, a post she held until 1979.
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All death files were confirmed in the local Catholic Church Burial Records.
All positions of sequence trace files were confirmed by manual inspection.
The specificity of primer sets designed for the qRT-PCR (Additional file 6) was confirmed by sequencing after the PCR reaction.
The occurrence of Helena in populations of D. simulans and D. mojavensis (Additional File 8) was confirmed by Southern blot using the detection system Gene Images Cdetectionetection module (Amersham Biosciences, Little Chalfont, UK).
The chicken PTH1R mRNA (Accession number FR746109, Additional file 1) was confirmed by RT-PCR on cDNA from whole chicken embryos at stage 26HH and from 3 ESTs found in database from stage 20-21HH whole chick embryos (BU219643), from stage 36HH limbs (BU401969) and from growth plate chondrocytes (BU419888).
Relaxed, a stealth startup centered around Apache CouchDB has raised $2 million from Redpoint Ventures according to an SEC filing which was confirmed by CEO Damien Katz.
The microarray data suggested that MLK1 is up-regulated in Ad5 E1-transformed cells compared to untransformed BRK cells by 2.39-fold (Additional file 1) which was confirmed by real-time PCR.
Bisulfite conversion efficiency for both, type I and II probes, was high for all tested samples (Additional file 4) and was confirmed by PCR using primers that are specific either to bisulfite converted or to non-modified GAPDH DNA regions (data not shown).
A saturation plot of the combined dataset (ingroup only, without taxa with missing one or more loci) did not show signs of substitution saturation (Additional file 1), which was confirmed by a statistical test for saturation (Iss = 0.645, Iss.cSym = 0.844, p < 0.0001) [ 53]. a Consistency Index.
Following induction, bacteriophage particles were visualized by transmission electron microscopy using negative staining with uranyl acetate (Additional file 16) and it was confirmed by the restriction analysis that they contain ϕPam-6 DNA.
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