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The RWA scaffold file was analyzed using first RepeatModeler [ 102] to identify RWA-specific repeats.
The alignment BAM file was analyzed using MACS v1.4 to detect read-enriched regions in the genome [ 41].
This file was analyzed using a locally installed Unix version of the InterProScan software [iprscan v4.6; InterPro release 24.0; accessed September, 15, 2010 (Quevillon et al. 2005; Hunter et al. 2009)] using default settings.
One quarter of the WGS assembly was masked, and the ".align" output file was analyzed using a custom Perl script, which removes hyper-mutable CpG sites and calculates distances from the consensus sequence using the Kimura 2-parameter model (Kimura 1980).
The data file was analyzed using a slightly modified version of model 2. We included the number of days postpartum that BCS was observed, and an interaction between days of observation postpartum and BCS to account for the biological changes due to fat mobilization early postpartum.
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The ASCC output file is analyzed using the maximum parsimony optimality criterion in TNT for 10,000 replications [116].
To confirm that post-burn-in trees represent the actual MCMC posterior distribution, marginal parameters (i.e., the MrBayes log file) were analyzed using the Effective Sample Size (ESS) statistic in the program Tracer [34].
The PKL files were analyzed using the MASCOT search engine.
Image files were analyzed using Hermes Dynamic Study display software V4.0 (Hermes Gold V2.10, Hermes Medical Solutions, Stockholm/London).
Data files were analyzed using FACSDiva™ software (BD).
The MGF files were analyzed using Proteome Discoverer 1.2 (Thermo Fisher Scientific).
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