Exact(2)
The bam file produced for quantification were first filtered to eliminate poorly aligned sequences using an alignment quality threshold of 30 and a PCR duplicate filtering step (samtools v. 0.1.19-44428 cd).
The same alignment file produced for the contig coverage calculations was parsed using an in-house script to obtain the proportion of the original genome's length covered by the longest aligning contig (maximum contig coverage).
Similar(58)
Passed Illumina reads were mapped to the final reference files produced for sugar beet and parsnip.
The expression index files produced for the different masking files were further analyzed for performance comparison and gene differential expression analysis using the Expressionist Analyst module.
Because the number of reads alone is not a good measure of which filter might be superior in producing sequence data suitable for phylogenetics, the files produced from each filter were examined for average read length and number of contigs assembled (table 1).
The user can also request that a file be produced for each knockout experiment that lists the fluxes of all the reactions in the corresponding reduced FBA model.
Reads were aligned to the GRC m38 (mouse mouse reference genome using NovoAlign-2.08.02 and a BAM file was produced for each strain (http://www.novocraft.com).
pHSY-1 and pHSY-2 files were produced for fourteen locations.
Two BED files were produced for use in genome browsers (one containing all transcripts and one with only large transcripts containing 3 or more exons) [ 31].
The distribution of Bayesian posterior probabilities for each clade was obtained by parsing MrBayes'trprob output files produced independently for each gene.
Alignment (.bam) files produced through mapping for gonads and brain were merged for each individual.
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