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The other four resources, such as the human reference genomic sequences (hg18), the mRNA/EST sequences, the 'gbCdnaInfo.txt' flat file (alignment between the human mRNAs/ESTs and genome sequences), and the 'tissue.txt' flat file (human mRNA/EST library information) were all downloaded from the UCSC genome browser website [42].
Supplementary sequence file, alignment PAML analysis, alignments S1 S4, references, and figures S1 S7 are available at Genome Biology and Evolution online (http://www.gbe.oxfordjournals.org/).org/
Alignment file (alignment between template and query) used for homology modeling (Fig. 4B) PDB file (output of MODELLER software after energy minimization) PYMOL session (pse) file, (PYMOL Molecular Graphics System, Version 1.2r3pre, Schrödinger, LLC) – The file contains the OR model wherein the predicted TM-helices are colored from 1 to 7 in VIBGYOR color for easy interpretation (Fig. 4C).
Although label-free approaches allow for flexible experimental designs, they are often associated with rigorous analytical and informatic requirements, including: attention to reproducibility of sample preparation and chromatographic separations; automation of multiple LC-MS file alignment; and automation of precursor ion detection and quantitation.
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These samples were converted into sequencing libraries using the double fragmentation approach and analyzed by AB/SOLiD sequencing (Table 1) (raw sequencing files, alignment files and called peaks have been submitted into GEO database with accession number: GSE18481).
All data files generated in the analysis, including sequence files, alignments, and networks, are available (http://babbittlab.compbio.ucsf.edu/resources/GST).
The website http://www.bioinf.uni-leipzig.de/Publications/SUPPLEMENTS/08-014 provides extensive machine readable information, including sequence files, alignments, and genomic coordinates.
12302_2016_97_MOESM2_ESM.tiff Additional file 2. Alignment of the 35S promoters from the CP4-epsps (Query) and cry1A.105 (Sbjct).
13568_2011_22_MOESM1_ESM.PDF Additional file 1: Alignment of proteins of the glycoside hydrolase family 32 (GH32) subfamilies from yeast species.
(PDF 155 KB) 13568_2011_22_MOESM2_ESM.PDF Additional file 2: Alignment of upstream sequences of inulinase genes from various strains of K. marxianus.
Additional file 10: EMF1 alignment.
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