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Additional file 3: Summary statistics for each FASTQ file aligned to the soybean reference genome.
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We first used samtools to extract the diploid genomes from their BAM files aligned to hg19, and excluded sex chromosomes and mitochondrial genomes because they are haploid.
We identified small insertions and deletions on the realigned, recalibrated pileup files (aligned to the N16961 reference genome) using Varscan, requiring a 75% variant frequency.
Single molecule maps in the adjusted merged BNX file are aligned to the in silico maps.
A FASTQ file was aligned to the sequences of target regions as described above, and the generated mapping data were processed to obtain the per base coverage (pileup files) using samtools.
The raw FASTQ read files were aligned to hg19 using the Isaac aligner to generate BAM files (Raczy et al., 2013).
Individual alignments are converted to FASTA files and aligned to an overall consensus.
35 bp sequencing reads and resulting FASTQ files were aligned to the chicken reference genome (galGal3) by the open-source aligner the Mapping and Assembly with Qualities (MAQ). 2 bp mismatches were allowed and retained uniquely mapped reads for further analysis.
Raw data from the Hiseq (fastq files) were aligned to ASM294v2 using Bowtie2 using default parameters.
The fastq files were aligned to both human (hg19) and mouse (mm10) genomes.
Fastq files were aligned to genomic repeats using Repeat Enrichment Estimator v1.0.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com