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We then used polarized fluorescence microscopy to detect potential asymmetries in the filament organization.
Altogether, our data suggest that MVT alters actin filament flexibility and tunes filament organization in the presence of VT.
In this manuscript, we present a minimalistic network Monte Carlo based approach to model actin filament organization under cyclic stretching.
These data provide the first in vivo evidence for the crucial role of a keratin tail domain in supramolecular keratin intermediate filament organization and barrier formation.
SMCs seeded on the ELP4 surface modified materials were also shown to exhibit the cell morphology and biological markers of a contractile phenotype including a spindle-like morphology, actin filament organization and smooth muscle myosin heavy chain expression.
Abnormal barrier function correlated with alterations in keratin filament organization (perinuclear retraction), impaired loading of lamellar body contents, followed by nonuniform extracellular distribution of secreted organelle contents, and abnormalities in lamellar bilayer architecture.
Results showed learning processes were associated with significant expression of genes, which were organized in several clusters of highly correlated genes and would be involved in biological processes such as intracellular signaling process, transcription regulation, and filament organization and axon guidance.
Nischarin alters actin filament organization and inhibits cell motility [42].
It has been suggested that interaction with myosin is critical for α-actin thin filament organization.
However, knockdown of smyhc1 had no effect on thick filament organization in fast muscles (Fig. 4E).
Similarly, inhibiting myosin ATPase with BTS also resulted in disruption of thick and thin filament organization.
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