Based on this new structure, we reanalyzed electron microscopy reconstructions of UvsX DNA filaments and docked the UvsX crystal structure into two different filament forms: a compressed filament generated in the presence of ADP and an elongated filament generated in the presence of ATP and aluminum fluoride.
Wu B, Peisley A, Li Z, Egelman E, Walz T, Penczek P, Hur S, 2014, 3.6 Angstrom resolution MAVS filament generated from helical reconstruction, http://www.ebi.ac.uk/pdbe/entry/EMD-5922, Publicly available at the Electron Microscopy Data Bank.
Wu B, Peisley A, Tetrault D, Li Z, Egelman EH, Magor KE, Walz T, Penczek PA, Hur S, 2014, 3.6 Angstrom resolution MAVS filament generated from helical reconstruction, http://www.rcsb.org/pdb/explore/explore.do?structureId=3j6j, Publicly available at RCSB Protein Data Bank.
When present at a very low concentration, Qdot 655 phalloidin bound stochastically to actin filaments, generating bright, photostable speckles along the actin filaments.
Numerous filaments generated around PC particles on the fracture surfaces, which bridged the PC particles and PE matrix, indicated a high interfacial adhesion between PC particles and PE matrix.
Unlike the branched network of short actin filaments generated by the Arp2/3 complex [5] [8], formin activity results in unbranched, long filaments in cells [9], [10].
Because actin monomers carry a functional polarity, actin filaments generate a functional polarity as well.
In contrast to the nuclear filaments generated by DMSO, the nuclear actin structures triggered by 0.01% MMS contained no detectable cofilin.
Moreover, the rank order of the diVCA construct nucleation activities was identical in the single-molecule measurements of the rate of branch formation observed on existing filaments and in bulk measurements of concentration of filaments generated in solution.
