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The total cellular mCherry signal was segmented into a diffuse and filament fraction using the Squassh segmentation tool from the Mosaic Suite of the Fiji image analysis software.
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After detection of heterocysts in the cultures 50 mL from each culture was harvested by centrifugation (5 min, 3500 g) and the pellet was resuspended in 500 μL denaturing alkylation buffer (DAB: 6 M Urea, 0.5% SDS, 10 mM EDTA in 200 mM Tris HCl pH 8.5) [ 19], frozen in liquid N2 and stored as the parental N2-fixing filament fractions at -80°C until further processing.
Iqbal et al. [ 60] used Gallyas method to stain the SDS-gel after electrophoresis of the paired helical filament (PHF) fraction extracted from AD brain.
Spinal cord filament samples (pellet fraction) were diluted 1∶20 with 1x loading buffer (50 mM Tris, 2% SDS, 10% glycerol, 100 mM DTT, 0.005% bromophenol blue).
The addition of 50 mmol/L human cardiac tropomyosin had very little effect on the fraction of filaments motile or filament sliding speed.
The previous paper discussed the relationship between fiber volume fraction in filament wound composite vessels and failure pressure.
AIP1 might be involved only in a fraction of filament severing events (Fig. 8A).
In contrast to the large majority of non-productive filament encounters, a small fraction of Arp2/3-diVCA filament binding events led to formation of a daughter filament.
For each Arp2/3 complex detected on the side of a tethered filament, we evaluated what fraction arrived complexed with diVCA (fAV).
So preservation that is good enough to record the impressions of scales on skin that are a few millimeter across, many not be fine enough to preserve tiny feather filaments that are a fraction of a millimeter across.
In wool, three cell types (ortho-, meso- and paracortex) contain macrofibrils with distinctly different filament arrangements and matrix fractions, but in human hair macrofibril-cell type relationships are less clear.
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