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Images and figures were analyzed with MPI (Siemens Corridor 4DM V501) and reviewed by a nuclear physician and cardiologist together.
After being rinsed with PBS another three times, cell sheets were observed with a fluorescence microscope (IX81, Olympus, Japan) and figures were analyzed with Image-Pro 6.0 software (Media Cybernetics, USA).
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The phosphorylation state of major thylakoid phosphoproteins was analyzed by immunoblotting with anti-phosphothreonine antibody (Figure S1).
The figures were analyzed by a University of Texas mathematics professor.
Y-981971 (Figure 1d), Y-793214 (Figure 1e), and Y-790782 (Figure 1f), were analyzed with a JEOL 8900 Electron Probe Microanalyzer (EPMA) (JEOL Ltd., Tokyo, Japan) at Atmosphere and Ocean Research Institute (AORI).
All other comparisons (except Figure 6 and Supplementary Figure 5) were analyzed with the unpaired, two-sided, independent Student's t-test without equal variance assumption.
A two-tailed unpaired Student's t-test was used for analysis of the experiments shown in Figure 2. Data from the experiments shown in Figure 1 were analyzed with ANOVA followed by Dunnett's Multiple Comparison Test.
The behavioral data in Figure 2A were analyzed with a two-tailed paired t test.
Image data were analyzed with BeadStudio V2.0.
The learning styles within each group are shown in Figure 2. The groups were analyzed with two-sample z test for two independent proportions.
Therefore, we also determined the expression of p63 and ki67 proliferation markers as well as p53 expression (Additional file 4: Figure S1), and they were analyzed with respect to VRK1 and VRK2 protein levels.
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