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A representative example is shown in the figure, and quantification of the data from six adhesions at the ends of ablated stress fibers from three different cells revealed 27,3 +/- 7,0 (SD)% decrease in tension at the area around focal adhesions.
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These results were confirmed also by histological analysis of Fabp4/aP2 expression (Figure 6C) and quantification of lipid accumulation (Figure 6D).
Analysis of serial dilutions of each infected sample by dot-blot (Additional file 3: Figure S2) and quantification of the signal using the Java image processing programe ImageJ indicated similar virus concentrations in leaves and the highest difference in cotyledons with a ratio of 7 considering the higher dilution of the virus (Additional file 4: Table S2).
Biotin was then detected in tumor tissue sections (Figure 1C), and quantification of biotin staining showed that significantly more DAB4-biotin than Sal5-biotin bound EL4 tumors in vivo, particularly in tumors of mice given chemotherapy (Figure 1D).
Figure 1 MRM and quantification of norbuprenorphine in urine extract.
Additional file 2: Figure S2 Detection and quantification of the novel m.12293G > A mutation.
Figure 1 Tumor concentration and quantification of sst 2 expression.
RNase L induction was monitored by binding to 2-5ApCp (Figure 1A and 1B) and quantification of the western blots indicated an increase in the concentration of RNase L compared to control C2-RNase L cells not treated with IPTG.
Furthermore Notch-1 IC protein expression in HMVEC was significantly decreased in the presence of DAPT (see online supplementary figure S2D). Figure 4A,B shows representative images and quantification of HMVEC invasion demonstrating significant EC invasion in response to VEGF and Ang2 alone, which was further potentiated by VEGF/Ang2 combination.
DOI: http://dx.doi.org/10.7554/eLife.01581.008 10.7554/eLiFigure81.009 Figure 3 figure supplement 2. Images and quantification of RNA interference (RNAi) degradation patterns.
Figure 1 shows representative images and quantification of the optimal vessel formation achieved when co-culturing BMEC or HUVEC with either hBMSC or hDF at the optimal endothelial:stromal cell ratio.
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