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Nuclei from 15 random fields were quantified using a 40× objective.
The ink particle-free tracks, produced by the migrating cells in the black ink assay, of five microscopic fields were quantified using ImageJ freeware.
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The area of CD31 positive fluorescent pixels across five random fields was quantified using Image J software and averaged for each of 8 tumors per experimental group.
Capillary-like tube structures per field were quantified using an inverted microscope (magnification ×100).
The spatial frequency of simulated EM field was quantified using Continuous Wavelet Transform (CWT) algorithm.
The area of tissue for each field was quantified using MetaMorph (Molecular Devices) by outlining tissue and calculating total area per field.
Each visual field was quantified using the following score: 0 5 cells = 0; 6–20 cells = 1; > 20 cells = 2.
The proportion of tumor area to the total tissue field was quantified using ImageJ with 5 fields analyzed for each section (at least 6 mice per group).
The area of the blood vessels in fields of equal area was quantified using the Image Pro Plus software.
Podoplanin positive lymphatic vessels in the diaphragms of 6 arbitrary 10x fields for each animal were quantified using the analysis program Image-J 1.37c (National Institute of Health, Bethesda, Maryland).
Four Ki67 labelled "hot spots" within selected high power fields of view (HPFs) were quantified using the NIS-Elements™ BR 3.1 software (Nikon Corporation, Tokyo, Japan), under 400× magnification.
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