We scored each tissue section for pPERK or peIF2α IHC staining according to the following scale: negative : no cells stained, rare (R): 1 3 cells stained, +: 4 20 cells stained, ++: 20+ cells stained, could have diffuse distribution of stained cells, may have high density of stained cells in some fields of the section, +++: high density of stained cells in almost every field of the section.
The basis functions are the fields of the sections of a magnetic system, and the coefficients of the expansion are the components of the magnetization vector of the sections (the vector synthesis).
Immunodetection of CD31 and DAPI staining were performed and random fields of the sections were photographed under fluorescence microscope.
Photos were taken under magnification of × 40 on four consecutive microscopic fields of the sections of implants.
So if place cell spiking activity represents connectivity, the place fields on the section of track replaced by the protrusion should extend continuously into the space provided by the protrusion.
All fields of the section were analyzed by two pathologists and more than 400 tumor cells, in five different area, were counted and mean percentage was calculated.
All staining was scored by counting the number of positive-stained cells, and was expressed as a percentage of the 1000 tumor cells counted across several representative fields of the section using a standard light microscope equipped with a × 100 square graticule.
Staining for bcl2, p53 and Ki67 was scored by counting the number of positively stained cells and was expressed as a percentage of the total tumour cells (at least 1,000) counted across several representative fields of the section using a standard light microscope equipped with a 10 × 10 square graticule.
Staining was scored by counting the number of positive-stained cells and expressed as a percentage of the total tumour cells (at least 1000) counted across several representative fields of the section using a standard light microscope equipped with a 10 × 10 square graticule.
For VEGF analysis, slides were first scanned at low magnification (×40), and five fields of the immunostained sections were randomly chosen for histomorphometry at ×400 magnification.
