Exact(9)
A ×20 dark field objective lens was used, giving a spot size of about 50 μmφ.
Measurements of visible reflectance spectra were performed by visible micro-spectroscopy (Yamanoi et al., 2009), which is composed of a visible-Raman microspectrometer (Yamanoi et al., 2004) with a dark field objective.
The number of MPI and MPII was determined by microscopic examination of the whole insert membrane; the results were expressed as the number of MPI or MPII per millimeter of well and on the width of the microscopic field (objective 100X) [4].
In the clinical field, objective and transparent evidence-based systematic review methods have been used for several decades.
Percentages were assessed by two independent observers, assuming that a high-power microscopic field (objective x40, microscopic magnification: x400) harbored 100 stromal cells (range: 75 150).
Optical dark-field images were captured in an Olympus BX51 upright microscope using a 100× dark field objective (Olympus LMPLFLN-BD, NA 0.8).
Similar(51)
However, imaging through a water air or glass air interface with high-NA reflected dark field objectives, which are not available as immersion objectives, usually creates high background scattering that degrades the NP film PNR signal.
Objective 1 Provide locally relevant and experiential educational opportunities for high school students and teachers, Objective 2 Support existing interest and increase confidence and enrollment in STEM fields, Objective 3 Stimulate interest in STEM through community engagement.
Focusing efforts on females and minorities but not excluding others the specific objectives of SMART are to: Objective 1 Provide locally relevant and experiential educational opportunities for high school students and teachers, Objective 2 Support existing interest and increase confidence and enrollment in STEM fields, Objective 3 Stimulate interest in STEM through community engagement.
A 100× dark-field objective (Olympus) with collection NA = 0.85 is used, providing excitation through an angular ring around 60° surrounding the collection cone.
Mitoses were counted from 10 consecutive fields (objective ×40, field diameter 450 μm).
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